ARG82834
Human PLA2G4A ELISA Kit
Human PLA2G4A ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82834-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82834-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82834-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82834-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82834-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82834-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82834-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82834-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82834-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82834-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82834-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82834 Human PLA2G4A ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human PLA2G4A in serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | PLA2G4A |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 31.3 pg/ml |
| Sample Type | Serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
| Standard Range | 62.5 - 4000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 4.8% Inter-Assay CV: 6.6% |
| Alternate Names | Phospholipase A2 group IVA; cPLA2; cPLA2-alpha; Phosphatidylcholine 2-acylhydrolase; PLA2G4; Cytosolic phospholipase A2; EC 3.1.1.5; EC 3.1.1.4 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PLA2G4A |
| Gene Full Name | phospholipase A2, group IVA (cytosolic, calcium-dependent) |
| Background | This gene encodes a member of the cytosolic phospholipase A2 group IV family. The enzyme catalyzes the hydrolysis of membrane phospholipids to release arachidonic acid which is subsequently metabolized into eicosanoids. Eicosanoids, including prostaglandins and leukotrienes, are lipid-based cellular hormones that regulate hemodynamics, inflammatory responses, and other intracellular pathways. The hydrolysis reaction also produces lysophospholipids that are converted into platelet-activating factor. The enzyme is activated by increased intracellular Ca(2+) levels and phosphorylation, resulting in its translocation from the cytosol and nucleus to perinuclear membrane vesicles. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015] |
| Function | Selectively hydrolyzes arachidonyl phospholipids in the sn-2 position releasing arachidonic acid. Together with its lysophospholipid activity, it is implicated in the initiation of the inflammatory response. [UniProt] |
| Cellular Localization | Cytoplasm. Cytoplasmic vesicle. Note=Translocates to membrane vesicles in a calcium-dependent fashion. [UniProt] |
| PTM | Activated by phosphorylation at both Ser-505 and Ser-727. [UniProt] |
Images (1) Click the Picture to Zoom In
-
ARG82834 Human PLA2G4A ELISA Kit standard curve image
ARG82834 Human PLA2G4A ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82834 Human PLA2G4A ELISA Kit User manual |
 Download
|
