ARG82749
Human PCOLCE / PCPE1 ELISA Kit
Human PCOLCE / PCPE1 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82749-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82749-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82749-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82749-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82749-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82749-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82749-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82749-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82749-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82749-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82749-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82749 Human PCOLCE / PCPE1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human PCOLCE / PCPE1 in serum, plasma (EDTA, heparin) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | PCOLCE / PCPE1 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 78 pg/ml |
| Sample Type | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.3% Inter-Assay CV: 6.2% |
| Alternate Names | Type I procollagen COOH-terminal proteinase enhancer; Procollagen C-endopeptidase enhancer 1; Procollagen C-proteinase enhancer 1; PCPE-1; Type 1 procollagen C-proteinase enhancer protein; PCPE1; Procollagen COOH-terminal proteinase enhancer 1; PCPE |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q15113 Human Procollagen C-endopeptidase enhancer 1 |
|---|---|
| Gene Symbol | PCOLCE |
| Gene Full Name | procollagen C-endopeptidase enhancer |
| Background | Fibrillar collagen types I-III are synthesized as precursor molecules known as procollagens. These precursors contain amino- and carboxyl-terminal peptide extensions known as N- and C-propeptides, respectively, which are cleaved, upon secretion of procollagen from the cell, to yield the mature triple helical, highly structured fibrils. This gene encodes a glycoprotein which binds and drives the enzymatic cleavage of type I procollagen and heightens C-proteinase activity. [provided by RefSeq, Jul 2008] |
| Function | Binds to the C-terminal propeptide of type I procollagen and enhances procollagen C-proteinase activity. C-terminal processed part of PCPE (CT-PCPE) may have an metalloproteinase inhibitory activity. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| Highlight | Related products: PCOLCE ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | C-terminally processed at multiple positions. [UniProt] |
Images (1) Click the Picture to Zoom In
-
ARG82749 Human PCOLCE / PCPE1 ELISA Kit standard curve image
ARG82749 Human PCOLCE / PCPE1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82749 Human PCOLCE / PCPE1 ELISA kit User manual |
 Download
|
