ARG82498
Human PAM / PAL ELISA Kit
Human PAM / PAL ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82498-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82498-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82498-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82498-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82498-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82498-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82498-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82498-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82498-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82498-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82498-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82498 Human PAM / PAL ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human PAM / PAL in serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | PAM / PAL |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 156 pg/ml |
| Sample Type | Serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
| Standard Range | 312 - 20000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 4.8% Inter-Assay CV: 6.7% |
| Alternate Names | Peptidylamidoglycolate lyase; PHM; EC 4.3.2.5; Peptidyl-glycine alpha-amidating monooxygenase; EC 1.14.17.3; PAL; PAM |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P19021 Human Peptidyl-glycine alpha-amidating monooxygenase |
|---|---|
| Gene Symbol | PAM |
| Gene Full Name | peptidylglycine alpha-amidating monooxygenase |
| Background | This gene encodes a multifunctional protein. It has two enzymatically active domains with catalytic activities - peptidylglycine alpha-hydroxylating monooxygenase (PHM) and peptidyl-alpha-hydroxyglycine alpha-amidating lyase (PAL). These catalytic domains work sequentially to catalyze neuroendocrine peptides to active alpha-amidated products. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene but some of their full length sequences are not yet known. [provided by RefSeq, Jul 2008] |
| Function | Bifunctional enzyme that catalyzes 2 sequential steps in C-terminal alpha-amidation of peptides. The monooxygenase part produces an unstable peptidyl(2-hydroxyglycine) intermediate that is dismutated to glyoxylate and the corresponding desglycine peptide amide by the lyase part. C-terminal amidation of peptides such as neuropeptides is essential for full biological activity. [UniProt] |
| Cellular Localization | Isoform 1: Membrane; Single-pass type I membrane protein. Isoform 2: Membrane; Single-pass type I membrane protein. Isoform 3: Secreted. Note=Secreted from secretory granules. Isoform 4: Secreted. Note=Secreted from secretory granules. [UniProt] |
| Highlight | Related products: PAM / PAL antibodies; PAM / PAL ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG82498 Human PAM / PAL ELISA Kit standard curve image
ARG82498 Human PAM / PAL ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82498 Human PAM / PAL ELISA Kit User manual |
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