ARG82415
Human P4HB ELISA Kit
Human P4HB ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82415-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82415-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82415-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82415-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82415-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82415-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82415-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82415-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82415-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82415-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82415-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82415 Human P4HB ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human P4HB in serum, plasma (EDTA, heparin) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | P4HB |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 46.9 pg/ml |
| Sample Type | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| Standard Range | 93.8 - 6000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.7% Inter-Assay CV: 6.5% |
| Alternate Names | Cellular thyroid hormone-binding protein; CLCRP1; PHDB; GIT; p55; P4Hbeta; PDIA1; ERBA2L; PROHB; PDI; DSI; Protein disulfide-isomerase; PO4DB; EC 5.3.4.1; Prolyl 4-hydroxylase subunit beta; PO4HB |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | P4HB |
| Gene Full Name | prolyl 4-hydroxylase, beta polypeptide |
| Background | This gene encodes the beta subunit of prolyl 4-hydroxylase, a highly abundant multifunctional enzyme that belongs to the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, this enzyme is involved in hydroxylation of prolyl residues in preprocollagen. This enzyme is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex. [provided by RefSeq, Jul 2008] |
| Function | This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP. [UniProt] |
| Cellular Localization | Endoplasmic reticulum lumen. Melanosome. Cell membrane; Peripheral membrane protein. Note=Highly abundant. In some cell types, seems to be also secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources. Localizes near CD4-enriched regions on lymphoid cell surfaces. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Colocalizes with MTTP in the endoplasmic reticulum. [UniProt] |
| Highlight | Related products: P4HB antibodies; P4HB ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG82415 Human P4HB ELISA Kit standard curve image
ARG82415 Human P4HB ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82415 Human P4HB ELISA Kit User manual |
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