ARG81417

Human Oncostatin M ELISA Kit

Human Oncostatin M ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG81417-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81417-002 Standard 2 X 10 ng/vial 4°C
ARG81417-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81417-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81417-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81417-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81417-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81417-008 25X Wash buffer 20 ml 4°C
ARG81417-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81417-010 STOP solution 10 ml (Ready to use) 4°C
ARG81417-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81417 Human Oncostatin M ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Oncostatin M in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name Oncostatin M
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 7.8 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 15.6 - 1000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 6.2%
Inter-Assay CV: 7.5%
Alternate Names OSM; Oncostatin-M

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 5008 Human OSM

Swiss-port # P13725 Human Oncostatin-M

Gene Symbol OSM
Gene Full Name oncostatin M
Background Oncostatin M is a member of a cytokine family that includes leukemia-inhibitory factor, granulocyte colony-stimulating factor, and interleukin 6. This gene encodes a growth regulator which inhibits the proliferation of a number of tumor cell lines. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells. [provided by RefSeq, Jul 2008]
Function Growth regulator. Inhibits the proliferation of a number of tumor cell lines. Stimulates proliferation of AIDS-KS cells. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells. Uses both type I OSM receptor (heterodimers composed of LIPR and IL6ST) and type II OSM receptor (heterodimers composed of OSMR and IL6ST). Involved in the maturation of fetal hepatocytes, thereby promoting liver development and regeneration (By similarity). [UniProt]
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PTM Propeptide processing is not important for receptor binding activity but may be important growth-inhibitory activity. [UniProt]

Images (1) Click the Picture to Zoom In

  • ARG81417 Human Oncostatin M ELISA Kit standard curve image

    ARG81417 Human Oncostatin M ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Title Download Link
ARG81417 Human Oncostatin M ELISA Kit User's manual download icon Download