ARG70454
Human OGG1 recombinant protein (His-tagged, C-ter)
Human OGG1 recombinant protein (His-tagged, C-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged (C-ter) Human OGG1 recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | OGG1 |
| Species | Human |
| A.A. Sequence | Met1 - Gly345 |
| Expression System | E. coli |
| Protein Full Name | N-glycosylase/DNA lyase |
| Alternate Names | OGG1; 8-Oxoguanine DNA Glycosylase; OGH1; MUTM; HOGG1; HMMH; 8-Hydroxyguanine DNA Glycosylase; N-Glycosylase/DNA Lyase; DNA-Apurinic Or Apyrimidinic Site Lyase |
Properties
| Form | Powder |
|---|---|
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | >98% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | OGG1 |
|---|---|
| Gene Full Name | 8-Oxoguanine DNA Glycosylase |
| Background | This gene encodes the enzyme responsible for the excision of 8-oxoguanine, a mutagenic base byproduct which occurs as a result of exposure to reactive oxygen. The action of this enzyme includes lyase activity for chain cleavage. Alternative splicing of the C-terminal region of this gene classifies splice variants into two major groups, type 1 and type 2, depending on the last exon of the sequence. Type 1 alternative splice variants end with exon 7 and type 2 end with exon 8. All variants share the N-terminal region in common, which contains a mitochondrial targeting signal that is essential for mitochondrial localization. Many alternative splice variants for this gene have been described, but the full-length nature for every variant has not been determined. |
| Function | DNA repair enzyme that incises DNA at 8-oxoG residues. Excises 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine (FAPY) from damaged DNA. Has a beta-lyase activity that nicks DNA 3' to the lesion. |
| Cellular Localization | Mitochondrion, Nucleus |
Images (1) Click the Picture to Zoom In
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ARG70454 Human OGG1 recombinant protein (His-tagged, C-ter) SDS-PAGE image
SDS-PAGE analysis of ARG70454 Human OGG1 recombinant protein (His-tagged, C-ter)
