ARG81575
Human NrCAM ELISA Kit
Human NrCAM ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81575-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81575-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81575-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81575-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81575-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81575-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81575-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81575-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81575-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81575-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81575-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81575 Human NrCAM ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human NrCAM in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | NrCAM |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 31.25 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 62.5 - 4000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 4.8% Inter-Assay CV: 6.1% |
| Alternate Names | Nr-CAM; Ng-CAM-related; hBravo; Neuronal surface protein Bravo; Neuronal cell adhesion molecule; NgCAM-related cell adhesion molecule |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | NRCAM |
| Gene Full Name | neuronal cell adhesion molecule |
| Background | Cell adhesion molecules (CAMs) are members of the immunoglobulin superfamily. This gene encodes a neuronal cell adhesion molecule with multiple immunoglobulin-like C2-type domains and fibronectin type-III domains. This ankyrin-binding protein is involved in neuron-neuron adhesion and promotes directional signaling during axonal cone growth. This gene is also expressed in non-neural tissues and may play a general role in cell-cell communication via signaling from its intracellular domain to the actin cytoskeleton during directional cell migration. Allelic variants of this gene have been associated with autism and addiction vulnerability. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
| Function | Cell adhesion, ankyrin-binding protein involved in neuron-neuron adhesion. May play a role in the molecular assembly of the nodes of Ranvier (By similarity). [UniProt] |
| Highlight | Related products: NrCAM antibodies; NrCAM ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
-
ARG81575 Human NrCAM ELISA Kit standard curve image
ARG81575 Human NrCAM ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81575 Human NrCAM ELISA Kit User's manual |
 Download
|
