ARG82466
Human Nephrin ELISA Kit
Human Nephrin ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82466-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82466-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82466-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82466-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82466-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82466-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82466-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82466-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82466-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82466-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82466-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82466 Human Nephrin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Nephrin in serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | Nephrin |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 78 pg/ml |
| Sample Type | Serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 4.4% Inter-Assay CV: 6.3% |
| Alternate Names | CNF; NPHN; Renal glomerulus-specific cell adhesion receptor; nephrin; Nephrin |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | NPHS1 |
| Gene Full Name | nephrosis 1, congenital, Finnish type (nephrin) |
| Background | This gene encodes a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. The gene is primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes. The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine. Mutations in this gene result in Finnish-type congenital nephrosis 1, characterized by severe proteinuria and loss of the slit diaphragm and foot processes.[provided by RefSeq, Oct 2009] |
| Function | Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion (By similarity). [UniProt] |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein. Note=Predominantly located at podocyte slit diaphragm between podocyte foot processes. Also associated with podocyte apical plasma membrane. [UniProt] |
| Highlight | Related products: Nephrin antibodies; Nephrin ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Phosphorylated at Tyr-1193 by FYN, leading to the recruitment and activation of phospholipase C-gamma-1/PLCG1. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG82466 Human Nephrin ELISA Kit standard curve image
ARG82466 Human Nephrin ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82466 Human Nephrin ELISA Kit User manual |
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