ARG82440
Human Nectin 3 ELISA Kit
Human Nectin 3 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82440-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82440-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82440-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82440-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82440-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82440-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82440-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82440-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82440-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82440-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82440-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82440 Human Nectin 3 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Nectin 3 in serum, plasma (EDTA, heparin) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | Nectin 3 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 62.5 pg/ml |
| Sample Type | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| Standard Range | 125 - 8000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.9% Inter-Assay CV: 5.3% |
| Alternate Names | PVRR3; PPR3; CDW113; PRR3; Nectin-3; CDw113; CD113; NECTIN-3; Poliovirus receptor-related protein 3; CD antigen CD113 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PVRL3 |
| Gene Full Name | poliovirus receptor-related 3 |
| Background | This gene encodes a member of the nectin family of proteins, which function as adhesion molecules at adherens junctions. This family member interacts with other nectin-like proteins and with afadin, a filamentous actin-binding protein involved in the regulation of directional motility, cell proliferation and survival. This gene plays a role in ocular development involving the ciliary body. Mutations in this gene are believed to result in congenital ocular defects. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2011] |
| Function | Plays a role in cell-cell adhesion through heterophilic trans-interactions with nectin-like proteins or nectins, such as trans-interaction with PVRL2/nectin-2 at Sertoli-spermatid junctions. Trans-interaction with PVR induces activation of CDC42 and RAC small G proteins through common signaling molecules such as SRC and RAP1. Also involved in the formation of cell-cell junctions, including adherens junctions and synapses. Induces endocytosis-mediated down-regulation of PVR from the cell surface, resulting in reduction of cell movement and proliferation. Plays a role in the morphology of the ciliary body. [UniProt] |
| Cellular Localization | Cell membrane; Single-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane. [UniProt] |
| Highlight | Related products: Nectin antibodies; Nectin ELISA Kits; Nectin Duos / Panels; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG82440 Human Nectin 3 ELISA Kit standard curve image
ARG82440 Human Nectin 3 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82440 Human Nectin 3 ELISA Kit User manual |
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