ARG83660
Human NUDT5 ELISA Kit
Human NUDT5 ELISA Kit for ELISA and Human
Overview
| Product Description | ARG83660 Human NUDT5 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human NUDT5 in Serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | NUDT5 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 50 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 93.7 - 6000 pg/ml |
| Sample Volume | 100 μl |
| Precision | Intra-Assay CV: < 10% Inter-Assay CV: < 10% |
| Alternate Names | NUDT5; Nudix Hydrolase 5; YSA1H; Nudix (Nucleoside Diphosphate Linked Moiety X)-Type Motif 5; Nuclear ATP-Synthesis Protein NUDIX5; ADP-Sugar Pyrophosphatase; 8-Oxo-DGDP Phosphatase; HYSAH1; HNUDT5; Nucleoside Diphosphate-Linked Moiety X Motif 5; Nudix Motif 5; EC 3.6.1.13; EC 3.6.1.58; EC 2.7.7.96; NUDIX5; YSAH1; YSA1 |
Application Instructions
| Assay Time | 4 hours |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 4°C, -20°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | NUDT5 |
| Gene Full Name | Nudix Hydrolase 5 |
| Background | This gene belongs to the Nudix (nucleoside diphosphate linked moiety X) hydrolase superfamily. The encoded enzyme catalyzes the hydrolysis of modified nucleoside diphosphates, including ADP-ribose (ADPR) and 8-oxoGua-containing 8-oxo-dADP and 8-oxo-dGDP. Protein-bound ADP ribose can be hazardous to the cell because it can modify some amino acid residues, resulting in the inhibition of ATP-activated potassium channels. 8-oxoGua is an oxidized form of guanine that can potentially alter genetic information by pairing with adenine and cytosine in RNA. Presence of 8-oxoGua in RNA results in formation of abnormal proteins due to translational errors. |
| Function | Enzyme that can either act as an ADP-sugar pyrophosphatase in absence of diphosphate or catalyze the synthesis of ATP in presence of diphosphate. [Uniprot] |
| Cellular Localization | Nucleus. [Uniprot] |
| PTM | Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation. [Uniprot] |
Images (1) Click the Picture to Zoom In
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ARG83660 Human NUDT5 ELISA Kit standard curve image
ARG83660 Human NUDT5 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
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| ARG83660 Human NUDT5 ELISA Kit User manual |
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