ARG83668
Human NQO1 ELISA Kit
Human NQO1 ELISA Kit for ELISA and Human
Overview
| Product Description | ARG83668 Human NQO1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human NQO1 in Serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | NQO1 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 10 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 15.6 - 1000 pg/ml |
| Sample Volume | 100 μl |
| Precision | Intra-Assay CV: < 10% Inter-Assay CV: < 10% |
| Alternate Names | NQO1; NAD(P)H Quinone Dehydrogenase 1; QR1; DTD; DT-Diaphorase; NMOR1; DHQU; DIA4; Diaphorase (NADH/NADPH) (Cytochrome B-5 Reductase); NAD(P)H Dehydrogenase [Quinone] 1; NAD(P)H Dehydrogenase, Quinone 1; NAD(P)H:Quinone Oxidoreductase 1; NAD(P)H-Quinone Oxidoreductase; Phylloquinone Reductase; Menadione Reductase; Quinone Reductase 1; Azoreductase; EC 1.6.5.2; NAD(P)H:Quinone Acceptor Oxidoreductase Type 1; NAD(P)H:Menadione Oxidoreductase 1; NAD(P)H:Quinone Oxireductase; Dioxin-Inducible 1; Quinone Reductase; Diaphorase-4; NMORI |
Application Instructions
| Assay Time | 4 hours |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 4°C, -20°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | NQO1 |
| Gene Full Name | NAD(P)H Quinone Dehydrogenase 1 |
| Background | This gene is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. This protein's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Mutations in this gene have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of this protein has been seen in many tumors and is also associated with Alzheimer's disease (AD). Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008] |
| Function | Acts as a gatekeeper of the core 20S proteasome known to degrade proteins with unstructured regions. Upon oxidative stress, interacts with tumor suppressors TP53 and TP73 in a NADH-dependent way and inhibits their ubiquitin-independent degradation by the 20S proteasome. [UniProt] |
| Cellular Localization | Cytoplasm. [UniProt] |
| PTM | Isopeptide bond, Phosphoprotein, Ubl conjugation. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG83668 Human NQO1 ELISA Kit standard curve image
ARG83668 Human NQO1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
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| ARG83668 Human NQO1 ELISA Kit User manual |
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