ARG83666
Human NOTCH3 ELISA Kit
Human NOTCH3 ELISA Kit for ELISA and Human
Overview
| Product Description | ARG83666 Human NOTCH3 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human NOTCH3 in Serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | NOTCH3 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 30 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 46.9 - 3000 pg/ml |
| Sample Volume | 100 μl |
| Precision | Intra-Assay CV: < 10% Inter-Assay CV: < 10% |
| Alternate Names | NOTCH3; Notch Receptor 3; Notch 3; CASIL; Neurogenic Locus Notch Homolog Protein 3; CADASIL; Notch (Drosophila) Homolog 3; Notch Homolog 3 (Drosophila); Notch Homolog 3; CADASIL1; IMF2; LMNS |
Application Instructions
| Assay Time | 4 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 4°C, -20°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9UM47 Human Neurogenic locus notch homolog protein 3 |
|---|---|
| Gene Symbol | NOTCH3 |
| Gene Full Name | Notch Receptor 3 |
| Background | This gene encodes the third discovered human homologue of the Drosophilia melanogaster type I membrane protein notch. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signalling pathway that plays a key role in neural development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remains to be determined. Mutations in NOTCH3 have been identified as the underlying cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL). [provided by RefSeq, Jul 2008] |
| Function | Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. [UniProt] |
| Cellular Localization | Cell membrane, Membrane, Nucleus. [UniProt] |
| PTM | Disulfide bond, Glycoprotein, Methylation, Phosphoprotein. [UniProt] |
Images (1) Click the Picture to Zoom In
-
ARG83666 Human NOTCH3 ELISA Kit standard curve image
ARG83666 Human NOTCH3 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG83666 Human NOTCH3 ELISA Kit User manual |
 Download
|
