ARG82430
Human NENF / Neudesin ELISA Kit
Human NENF / Neudesin ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82430-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82430-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82430-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82430-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82430-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82430-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82430-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82430-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82430-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82430-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82430-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82430 Human NENF / Neudesin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human NENF / Neudesin in serum, plasma (EDTA, heparin) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | NENF / Neudesin |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 46.8 pg/ml |
| Sample Type | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| Standard Range | 93.7 - 6000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.5% Inter-Assay CV: 5.7% |
| Alternate Names | CIR2; Protein GIG47; SPUF protein; Cell immortalization-related protein 2; SPUF; Neuron-derived neurotrophic factor; SCIRP10; Neudesin; Secreted protein of unknown function |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | NENF |
| Gene Full Name | neudesin neurotrophic factor |
| Background | This gene encodes a neurotrophic factor that may play a role in neuron differentiation and development. A pseudogene of this gene is found on chromosome 12. Alternate splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2009] |
| Function | Acts as a neurotrophic factor in postnatal mature neurons enhancing neuronal survival. Promotes cell proliferation and neurogenesis in undifferentiated neural pro-genitor cells at the embryonic stage and inhibits differentiation of astrocyte. Its neurotrophic activity is exerted via MAPK1/ERK2, MAPK3/ERK1 and AKT1/AKT pathways. Neurotrophic activity is enhanced by binding to heme. Acts also as an anorexigenic neurotrophic factor that contributes to energy balance (By similarity). Plays a role in the human tumorigenesis. [UniProt] |
| Cellular Localization | Secreted, extracellular space. [UniProt] |
| Highlight | Related products: New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG82430 Human NENF / Neudesin ELISA Kit standard curve image
ARG82430 Human NENF / Neudesin ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82430 Human NENF / Neudesin ELISA Kit User manual |
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