ARG81602
Human NECTIN4 / PVRL4 ELISA Kit
Human NECTIN4 / PVRL4 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81602-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81602-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81602-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81602-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81602-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81602-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81602-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81602-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81602-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81602-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81602-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81602 Human NECTIN4 / PVRL4 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human NECTIN4 / PVRL4 in serum, plasma (heparin, EDTA), urine and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | NECTIN4 / PVRL4 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 15.6 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA), urine and cell culture supernatants. |
| Standard Range | 31.2 - 2000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.5% Inter-Assay CV: 6.1% |
| Alternate Names | Nectin-4; Ig superfamily receptor LNIR; PRR4; LNIR; nectin-4; EDSS1; Poliovirus receptor-related protein 4; Nectin cell adhesion molecule 4 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PVRL4 |
| Gene Full Name | poliovirus receptor-related 4 |
| Background | This gene encodes a member of the nectin family. The encoded protein contains two immunoglobulin-like (Ig-like) C2-type domains and one Ig-like V-type domain. It is involved in cell adhesion through trans-homophilic and -heterophilic interactions. It is a single-pass type I membrane protein. The soluble form is produced by proteolytic cleavage at the cell surface by the metalloproteinase ADAM17/TACE. The secreted form is found in both breast tumor cell lines and breast tumor patients. Mutations in this gene are the cause of ectodermal dysplasia-syndactyly syndrome type 1, an autosomal recessive disorder. Alternatively spliced transcript variants have been found but the full-length nature of the variant has not been determined.[provided by RefSeq, Jan 2011] |
| Function | Seems to be involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with PVRL2/nectin-1. Does not act as receptor for alpha-herpesvirus entry into cells. [UniProt] |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein. Cell junction, adherens junction. Note=Colocalizes with AFDN at cadherin-based adherens junctions (PubMed:11544254). Processed poliovirus receptor-related protein 4: Secreted. Note=The secreted form is found in breast tumor patients (PubMed:15784625). [UniProt] |
| Highlight | New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | The soluble form is produced by proteolytic cleavage at the cell surface (shedding), probably by ADAM17/TACE. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81602 Human NECTIN4 / PVRL4 ELISA Kit standard curve image
ARG81602 Human NECTIN4 / PVRL4 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81602 Human PVRL4 ELISA Kit User's manual |
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