ARG81447
Human NBL1 ELISA Kit
Human NBL1 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81447-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81447-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81447-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81447-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81447-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81447-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81447-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81447-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81447-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81447-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81447-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81447 Human NBL1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human NBL1 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | NBL1 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 62.5 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 125 - 8000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.3% Inter-Assay CV: 6.6% |
| Alternate Names | NB; DAN; NO3; DAND1; D1S1733E |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P41271 Human Neuroblastoma suppressor of tumorigenicity 1 |
|---|---|
| Gene Symbol | NBL1 |
| Gene Full Name | NBL1, DAN family BMP antagonist |
| Background | This gene product is the founding member of the evolutionarily conserved CAN (Cerberus and DAN) family of proteins, which contain a domain resembling the CTCK (C-terminal cystine knot-like) motif found in a number of signaling molecules. These proteins are secreted, and act as BMP (bone morphogenetic protein) antagonists by binding to BMPs and preventing them from interacting with their receptors. They may thus play an important role during growth and development. Alternatively spliced transcript variants have been identified for this gene. Read-through transcripts between this locus and the upstream mitochondrial inner membrane organizing system 1 gene (GeneID 440574) have been observed. [provided by RefSeq, May 2013] |
| Function | Possible candidate as a tumor suppressor gene of neuroblastoma. May play an important role in preventing cells from entering the final stage (G1/S) of the transformation process. [UniProt] |
| Highlight | Related products: NBL1 antibodies; NBL1 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG81447 Human NBL1 ELISA Kit standard curve image
ARG81447 Human NBL1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81447 Human NBL1 ELISA Kit User's manual |
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