ARG83653

Human NAAA ELISA Kit

Human NAAA ELISA Kit for ELISA and Human

Overview

Product Description ARG83653 Human NAAA ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human NAAA in serum, plasma (heparin, EDTA, citrate) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Target Name NAAA
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 10 pg/ml
Sample Type Serum, plasma (heparin, EDTA, citrate) and cell culture supernatants.
Standard Range 15.6 - 1000 pg/ml
Sample Volume 100 μl
Precision Intra-Assay CV: < 10%
Inter-Assay CV: < 10%
Alternate Names NAAA; N-Acylethanolamine Acid Amidase; ASAHL; N-Acylethanolamine-Hydrolyzing Acid Amidase; Acylsphingosine Deacylase NAAA; Acid Ceramidase-Like Protein; ASAH-Like Protein; PLT; N-Acylsphingosine Amidohydrolase (Acid Ceramidase)-Like; N-Acylsphingosine Amidohydrolase-Like; EC 3.5.1.60; EC 3.5.1.23

Application Instructions

Assay Time 4 hours

Properties

Form 96 well
Storage Instruction Store the kit at 4°C, -20°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 27163 Human NAAA

Swiss-port # Q02083 Human N-acylethanolamine-hydrolyzing acid amidase

Gene Symbol NAAA
Gene Full Name N-Acylethanolamine Acid Amidase
Background Enables DNA-binding transcription factor binding activity and hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides. Involved in several processes, including N-acylethanolamine metabolic process; N-acylphosphatidylethanolamine metabolic process; and sphingosine metabolic process. Located in lysosome and membrane. [provided by Alliance of Genome Resources, Nov 2024]
Function Also exhibits weak hydrolytic activity against the ceramides N-lauroylsphingosine and N-palmitoylsphingosine. [Uniprot]
Cellular Localization Lysosome, Membrane. [Uniprot]
PTM Autocatalytic cleavage, Disulfide bond, Glycoprotein, Zymogen. [Uniprot]

Images (1) Click the Picture to Zoom In

  • ARG83653 Human NAAA ELISA Kit standard curve image

    ARG83653 Human NAAA ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Title Download Link
ARG83653 Human NAAA ELISA Kit User manual download icon Download