ARG83654
Human Myocilin ELISA Kit
Human Myocilin ELISA Kit for ELISA and Human
Overview
| Product Description | ARG83654 Human Myocilin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Myocilin in serum, plasma (heparin, EDTA) and cell culture supernatants. |
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| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | Myocilin |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 100 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 μl |
| Precision | Intra-Assay CV: < 10% Inter-Assay CV: < 10% |
| Alternate Names | MYOC; Myocilin; TIGR; JOAG1; GLC1A; Trabecular Meshwork Inducible Glucocorticoid Response Protein; Juvenile-Onset Open-Angle Glaucoma 1; Myocilin 55 KDa Subunit; Myocilin Trabecular Meshwork Inducible Glucocorticoid Response Protein; Mutated Trabecular Meshwork-Induced Glucocorticoid Response Protein; Myocilin, Trabecular Meshwork Inducible Glucocorticoid Response; Trabecular Meshwork-Induced Glucocorticoid Response Protein; Myocilin Protein; GPOA; JOAG |
Application Instructions
| Assay Time | 4 hours |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 4°C, -20°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | MYOC |
| Gene Full Name | Myocilin |
| Background | MYOC encodes the protein myocilin, which is believed to have a role in cytoskeletal function. MYOC is expressed in many occular tissues, including the trabecular meshwork, and was revealed to be the trabecular meshwork glucocorticoid-inducible response protein (TIGR). The trabecular meshwork is a specialized eye tissue essential in regulating intraocular pressure, and mutations in MYOC have been identified as the cause of hereditary juvenile-onset open-angle glaucoma. [provided by RefSeq, Jul 2008] |
| Function | Secreted glycoprotein regulating the activation of different signaling pathways in adjacent cells to control different processes including cell adhesion, cell-matrix adhesion, cytoskeleton organization and cell migration. Promotes substrate adhesion, spreading and formation of focal contacts. Negatively regulates cell-matrix adhesion and stress fiber assembly through Rho protein signal transduction. Modulates the organization of actin cytoskeleton by stimulating the formation of stress fibers through interactions with components of Wnt signaling pathways. Promotes cell migration through activation of PTK2 and the downstream phosphatidylinositol 3-kinase signaling. Plays a role in bone formation and promotes osteoblast differentiation in a dose-dependent manner through mitogen-activated protein kinase signaling. Mediates myelination in the peripheral nervous system through ERBB2/ERBB3 signaling. Plays a role as a regulator of muscle hypertrophy through the components of dystrophin-associated protein complex. Involved in positive regulation of mitochondrial depolarization. Plays a role in neurite outgrowth. May participate in the obstruction of fluid outflow in the trabecular meshwork. [Uniprot] |
| Cellular Localization | Cell projection, Cilium, Cytoplasmic vesicle, Endoplasmic reticulum, Extracellular matrix, Golgi apparatus, Membrane, Mitochondrion, Mitochondrion inner membrane, Mitochondrion outer membrane, Secreted |
| PTM | Disulfide bond, Glycoprotein, Lipoprotein, Palmitate. [Uniprot] |
Images (1) Click the Picture to Zoom In
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ARG83654 Human Myocilin ELISA Kit standard curve image
ARG83654 Human Myocilin ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
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| ARG83654 Human Myocilin ELISA Kit User manual |
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