ARG82847
Human MyD88 ELISA Kit
Human MyD88 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82847-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82847-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82847-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82847-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82847-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82847-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82847-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82847-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82847-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82847-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82847-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82847 Human MyD88 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human MyD88 in serum, plasma (EDTA, heparin), cell culture supernatants and bone tissue. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | MyD88 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 78 pg/ml |
| Sample Type | Serum, plasma (EDTA, heparin), cell culture supernatants and bone tissue. |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.7% Inter-Assay CV: 8.0% |
| Alternate Names | MYD88D; Myeloid differentiation primary response protein MyD88 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q99836 Human Myeloid differentiation primary response protein MyD88 |
|---|---|
| Gene Symbol | MYD88 |
| Gene Full Name | myeloid differentiation primary response 88 |
| Background | This gene encodes a cytosolic adapter protein that plays a central role in the innate and adaptive immune response. This protein functions as an essential signal transducer in the interleukin-1 and Toll-like receptor signaling pathways. These pathways regulate that activation of numerous proinflammatory genes. The encoded protein consists of an N-terminal death domain and a C-terminal Toll-interleukin1 receptor domain. Patients with defects in this gene have an increased susceptibility to pyogenic bacterial infections. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2010] |
| Function | Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response (PubMed:15361868, PubMed:18292575). Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed:15361868, PubMed:24316379, PubMed:19506249). Increases IL-8 transcription (PubMed:9013863). Involved in IL-18-mediated signaling pathway. Activates IRF1 resulting in its rapid migration into the nucleus to mediate an efficient induction of IFN-beta, NOS2/INOS, and IL12A genes. MyD88-mediated signaling in intestinal epithelial cells is crucial for maintenance of gut homeostasis and controls the expression of the antimicrobial lectin REG3G in the small intestine (By similarity). [UniProt] |
| Cellular Localization | Cytoplasm. Nucleus. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG82847 Human MyD88 ELISA Kit standard curve image
ARG82847 Human MyD88 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82847 Human MyD88 ELISA Kit User manual |
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