ARG81446
Human Met ELISA Kit
Human Met ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81446-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81446-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81446-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81446-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81446-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81446-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81446-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81446-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81446-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81446-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81446-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81446 Human Met ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Met in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | Met |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 31.25 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 62.5 - 4000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.2% Inter-Assay CV: 6.1% |
| Alternate Names | Scatter factor receptor; c-Met; HGF receptor; HGFR; EC 2.7.10.1; SF receptor; AUTS9; Proto-oncogene c-Met; Tyrosine-protein kinase Met; HGF/SF receptor; Hepatocyte growth factor receptor; RCCP2; DFNB97 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | MET |
| Gene Full Name | MET proto-oncogene, receptor tyrosine kinase |
| Background | The proto-oncogene MET product is the hepatocyte growth factor receptor and encodes tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Various mutations in the MET gene are associated with papillary renal carcinoma. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Function | Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to hepatocyte growth factor/HGF ligand. Regulates many physiological processes including proliferation, scattering, morphogenesis and survival. Ligand binding at the cell surface induces autophosphorylation of MET on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1. Recruitment of these downstream effectors by MET leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. The RAS-ERK activation is associated with the morphogenetic effects while PI3K/AKT coordinates prosurvival effects. During embryonic development, MET signaling plays a role in gastrulation, development and migration of muscles and neuronal precursors, angiogenesis and kidney formation. In adults, participates in wound healing as well as organ regeneration and tissue remodeling. Promotes also differentiation and proliferation of hematopoietic cells. Acts as a receptor for Listeria internalin inlB, mediating entry of the pathogen into cells. [UniProt] |
| Highlight | Related products: Met antibodies; Met ELISA Kits; Met Duos / Panels; Met recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Autophosphorylated in response to ligand binding on Tyr-1234 and Tyr-1235 in the kinase domain leading to further phosphorylation of Tyr-1349 and Tyr-1356 in the C-terminal multifunctional docking site. Dephosphorylated by PTPRJ at Tyr-1349 and Tyr-1365. Dephosphorylated by PTPN1 and PTPN2. Ubiquitinated. Ubiquitination by CBL regulates MET endocytosis, resulting in decreasing plasma membrane receptor abundance, and in endosomal degradation and/or recycling of internalized receptors. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81446 Human Met ELISA Kit standard curve image
ARG81446 Human Met ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81446 Human Met ELISA Kit User's manual |
 Download
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