ARG70514
Human Mesothelin recombinant protein (His-tagged)
Human Mesothelin recombinant protein (His-tagged) for SDS-PAGE
Overview
| Product Description | CHO expressed, His-tagged Human Mesothelin recombinant protein. |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | Mesothelin |
| Species | Human |
| A.A. Sequence | Glu296-Gly580 |
| Expression System | CHO |
| Protein Full Name | Mesothelin |
| Alternate Names | MSLN; Mesothelin; MPF; Pre-Pro-Megakaryocyte-Potentiating Factor; CAK1 Antigen; CAK1; Soluble MPF Mesothelin Related Protein; Megakaryocyte Potentiating Factor; SMRP |
Properties
| Form | Powder |
|---|---|
| Purification | >95% (by SDS-PAGE) |
| Purification Note | Endotoxin level is <0.1 EU/µg of the protein, as determined by the LAL test. |
| Buffer | PBS (pH 7.4) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in 4 mM HCl to a concentration not <200 μg/mL and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | MSLN |
|---|---|
| Gene Full Name | Mesothelin |
| Background | This gene encodes a preproprotein that is proteolytically processed to generate two protein products, megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiating factor functions as a cytokine that can stimulate colony formation of bone marrow megakaryocytes. Mesothelin is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Feb 2016] |
| Function | Membrane-anchored forms may play a role in cellular adhesion. Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro. [UniProt] |
