ARG81556
Human Mesothelin ELISA Kit
Human Mesothelin ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81556-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81556-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81556-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81556-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81556-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81556-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81556-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81556-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81556-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81556-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81556-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81556 Human Mesothelin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Mesothelin in serum, plasma (heparin, EDTA), saliva, urine and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | Mesothelin |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 78 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA), saliva, urine and cell culture supernatants. |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.4% Inter-Assay CV: 6.6% |
| Alternate Names | MPF; Mesothelin; CAK1 antigen; Pre-pro-megakaryocyte-potentiating factor; SMRP |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | MSLN |
| Gene Full Name | mesothelin |
| Background | This gene encodes a precursor protein that is cleaved into two products, megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiation factor functions as a cytokine that can stimulate colony formation in bone marrow megakaryocytes. Mesothelian is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Apr 2010] |
| Function | Membrane-anchored forms may play a role in cellular adhesion. Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro. [UniProt] |
| Highlight | Related products: Mesothelin antibodies; Mesothelin ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Both MPF and the cleaved form of mesothelin are N-glycosylated. Proteolytically cleaved by a furin-like convertase to generate megakaryocyte-potentiating factor (MPF), and the cleaved form of mesothelin. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81556 Human Mesothelin ELISA Kit standard curve image
ARG81556 Human Mesothelin ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81556 Human Mesothelin ELISA Kit User manual |
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