ARG80644
Human MUC1 / EMA ELISA Kit
Human MUC1 / EMA ELISA Kit for ELISA and Human
Cancer kit; Controls and Markers kit; Signaling Transduction kit
Overview
| Product Description | ARG80644 Human MUC1 / EMA ELISA Kit is an enzyme immunoassay kit for the quantification of Human MUC1 / EMA (CA15-3) in serum and plasma (EDTA, heparin, citrate). |
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| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | MUC1 / EMA |
| Sensitivity | 0.5 U/ml |
| Sample Type | Serum and plasma (EDTA, heparin, citrate). |
| Standard Range | 25 - 200 U/ml |
| Sample Volume | 10 µl |
| Alternate Names | MUC1-NT; EMA; Mucin-1; MAM6; PEM; Peanut-reactive urinary mucin; CD227; MUC-1/SEC; Breast carcinoma-associated antigen DF3; MUC-1/X; Cancer antigen 15-3; H23AG; CD antigen CD227; MCKD; Carcinoma-associated mucin; Polymorphic epithelial mucin; MUC-1; MUC1-alpha; KL-6; Tumor-associated epithelial membrane antigen; MUC1-CT; ADMCKD1; Episialin; PUM; Tumor-associated mucin; PEMT; MCKD1; ADMCKD; MCD; Krebs von den Lungen-6; CA 15-3; MUC1-beta; MUC1/ZD |
Application Instructions
| Assay Time | 60, 60, 15 min |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
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| Gene Symbol | MUC1 |
| Gene Full Name | mucin 1, cell surface associated |
| Background | This gene encodes a membrane-bound protein that is a member of the mucin family. Mucins are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces. These proteins also play a role in intracellular signaling. This protein is expressed on the apical surface of epithelial cells that line the mucosal surfaces of many different tissues including lung, breast stomach and pancreas. This protein is proteolytically cleaved into alpha and beta subunits that form a heterodimeric complex. The N-terminal alpha subunit functions in cell-adhesion and the C-terminal beta subunit is involved in cell signaling. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. This gene is known to contain a highly polymorphic variable number tandem repeats (VNTR) domain. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Feb 2011] |
| Function | The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack. The beta subunit contains a C-terminal domain which is involved in cell signaling, through phosphorylations and protein-protein interactions. Modulates signaling in ERK, SRC and NF-kappa-B pathways. In activated T-cells, influences directly or indirectly the Ras/MAPK pathway. Promotes tumor progression. Regulates TP53-mediated transcription and determines cell fate in the genotoxic stress response. Binds, together with KLF4, the PE21 promoter element of TP53 and represses TP53 activity. [UniProt] |
| Highlight | Related products: MUC1 antibodies; MUC1 ELISA Kits; MUC1 Duos / Panels; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Research Area | Cancer kit; Controls and Markers kit; Signaling Transduction kit |
| PTM | Highly glycosylated (N- and O-linked carbohydrates and sialic acid). O-glycosylated to a varying degree on serine and threonine residues within each tandem repeat, ranging from mono- to penta-glycosylation. The average density ranges from about 50% in human milk to over 90% in T47D breast cancer cells. Further sialylation occurs during recycling. Membrane-shed glycoproteins from kidney and breast cancer cells have preferentially sialyated core 1 structures, while secreted forms from the same tissues display mainly core 2 structures. The O-glycosylated content is overlapping in both these tissues with terminal fucose and galactose, 2- and 3-linked galactose, 3- and 3,6-linked GalNAc-ol and 4-linked GlcNAc predominating. Differentially O-glycosylated in breast carcinomas with 3,4-linked GlcNAc. N-glycosylation consists of high-mannose, acidic complex-type and hybrid glycans in the secreted form MUC1/SEC, and neutral complex-type in the transmembrane form, MUC1/TM. Proteolytic cleavage in the SEA domain occurs in the endoplasmic reticulum by an autoproteolytic mechanism and requires the full-length SEA domain as well as requiring a Ser, Thr or Cys residue at the P + 1 site. Cleavage at this site also occurs on isoform MUC1/X but not on isoform MUC1/Y. Ectodomain shedding is mediated by ADAM17. Dual palmitoylation on cysteine residues in the CQC motif is required for recycling from endosomes back to the plasma membrane. Phosphorylated on tyrosines and serine residues in the C-terminal. Phosphorylation on tyrosines in the C-terminal increases the nuclear location of MUC1 and beta-catenin. Phosphorylation by PKC delta induces binding of MUC1 to beta-catenin/CTNNB1 and thus decreases the formation of the beta-catenin/E-cadherin complex. Src-mediated phosphorylation inhibits interaction with GSK3B. Src- and EGFR-mediated phosphorylation on Tyr-1229 increases binding to beta-catenin/CTNNB1. GSK3B-mediated phosphorylation on Ser-1227 decreases this interaction but restores the formation of the beta-cadherin/E-cadherin complex. On T-cell receptor activation, phosphorylated by LCK. PDGFR-mediated phosphorylation increases nuclear colocalization of MUC1CT and CTNNB1. The N-terminal sequence has been shown to begin at position 24 or 28. |
Images (1) Click the Picture to Zoom In
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ARG80644 Human MUC1 / EMA ELISA Kit standard curve image
ARG80644 Human MUC1 / EMA ELISA Kit Results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
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| ARG80644 Human MUC1 / EMA ELISA Kit User's manual |
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