ARG82072
Human MPO / Myeloperoxidase ELISA Kit
Human MPO / Myeloperoxidase ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82072-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82072-002 | Standard | 2 X 20 ng/vial | 4°C |
| ARG82072-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82072-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82072-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82072-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82072-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82072-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82072-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82072-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82072-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82072 Human MPO / Myeloperoxidase ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human MPO / Myeloperoxidase in serum, plasma (heparin, EDTA), saliva, urine and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | MPO / Myeloperoxidase |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 156 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA), saliva, urine and cell culture supernatants. |
| Standard Range | 312 - 20000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.1%; Inter-Assay CV: 7.2% |
| Alternate Names | MPO; Myeloperoxidase; EC 1.11.2.2 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | MPO |
| Gene Full Name | myeloperoxidase |
| Background | Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of neutrophils. [provided by RefSeq, Nov 2014] |
| Function | Myeloperoxidase (MPO): Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. [UniProt] |
| Highlight | Related products: MPO antibodies; MPO ELISA Kits; MPO Duos / Panels; Related news: Exploring Antiviral Immune Response New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG82072 Human MPO / Myeloperoxidase ELISA Kit standard curve image
ARG82072 Human MPO / Myeloperoxidase ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82072 Human MPO / Myeloperoxidase ELISA Kit User's manual |
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