ARG81435
Human MIP4 ELISA Kit
Human MIP4 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81435-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81435-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81435-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81435-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81435-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81435-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81435-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81435-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81435-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81435-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81435-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81435 Human MIP4 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human MIP4 in serum and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | MIP4 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 3.9 pg/ml |
| Sample Type | Serum and cell culture supernatants. |
| Standard Range | 7.8 - 500 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.7% Inter-Assay CV: 8.1% |
| Alternate Names | Small-inducible cytokine A18; 3-69; PARC; Alternative macrophage activation-associated CC chemokine 1; Macrophage inflammatory protein 4; SCYA18; Dendritic cell chemokine 1; CC chemokine PARC; 4-69; 1-68; C-C motif chemokine 18; DC-CK1; AMAC1; Pulmonary and activation-regulated chemokine; AMAC-1; DCCK1; MIP-4; CKb7 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CCL18 |
| Gene Full Name | chemokine (C-C motif) ligand 18 (pulmonary and activation-regulated) |
| Background | This antimicrobial gene is one of several Cys-Cys (CC) cytokine genes clustered on the q arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for naive T cells, CD4+ and CD8+ T cells and nonactivated lymphocytes, but not for monocytes or granulocytes. This chemokine attracts naive T lymphocytes toward dendritic cells and activated macrophages in lymph nodes. It may play a role in both humoral and cell-mediated immunity responses. [provided by RefSeq, Sep 2014] |
| Function | Chemotactic factor that attracts lymphocytes but not monocytes or granulocytes. May be involved in B-cell migration into B-cell follicles in lymph nodes. Attracts naive T-lymphocytes toward dendritic cells and activated macrophages in lymph nodes, has chemotactic activity for naive T-cells, CD4+ and CD8+ T-cells and thus may play a role in both humoral and cell-mediated immunity responses. [UniProt] |
| Highlight | Related products: MIP4 antibodies; MIP4 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | The Cys-30/Cys-54 disulfide bond is required for activity. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81435 Human MIP4 ELISA Kit standard curve image
ARG81435 Human MIP4 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81435 Human MIP4 ELISA Kit User's manual |
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