ARG70389

Human MIF recombinant protein (His-tagged, C-ter)

Human MIF recombinant protein (His-tagged, C-ter) for SDS-PAGE

Overview

Product Description E. coli expressed, His-tagged (C-ter) Human MIF recombinant protein
Tested Application SDS-PAGE
Target Name MIF
Species Human
A.A. Sequence Met1 - Ala115
Expression System E. coli
Protein Full Name Macrophage migration inhibitory factor
Alternate Names MIF; Macrophage Migration Inhibitory Factor; GIF; Phenylpyruvate Tautomerase; GLIF; Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor); Glycosylation-Inhibiting Factor; L-Dopachrome Tautomerase; L-Dopachrome Isomerase; MMIF; Epididymis Secretory Sperm Binding Protein; EC 5.3.3.12; EC 5.3.2.1

Properties

Form Powder
Purification Note Endotoxin level is <0.1 EU/µg of the protein, as determined by the LAL test.
Purity >98% (by SDS-PAGE)
Buffer PBS (pH 8.0)
Reconstitution It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not <200 μg/mL and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely.
Storage Instruction For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol MIF
Gene Full Name Macrophage Migration Inhibitory Factor
Background This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways.
Function It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.
Cellular Localization Cytoplasm, Secreted

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  • ARG70389 Human MIF recombinant protein (His-tagged, C-ter) SDS-PAGE image

    SDS-PAGE analysis of  ARG70389 Human MIF recombinant protein (His-tagged, C-ter)