ARG81294
Human MIF ELISA Kit
Human MIF ELISA Kit for ELISA and Human
Publication1
Overview
| Product Description | ARG81294 Human MIF ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human MIF in serum, plasma (platelet free) and cell culture supernatants |
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| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | MIF |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 15 pg/ml |
| Sample Type | serum, plasma (platelet free) and cell culture supernatants |
| Standard Range | 31.25 - 2000 pg/ml |
| Sample Volume | 100 µl |
| Alternate Names | Phenylpyruvate tautomerase; L-dopachrome tautomerase; GLIF; L-dopachrome isomerase; MMIF; Macrophage migration inhibitory factor; MIF; GIF; EC 5.3.2.1; Glycosylation-inhibiting factor; EC 5.3.3.12 |
Application Instructions
| Assay Time | ~ 3.5 hours |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P14174 Human Macrophage migration inhibitory factor |
|---|---|
| Gene Symbol | MIF |
| Gene Full Name | macrophage migration inhibitory factor (glycosylation-inhibiting factor) |
| Background | This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. [provided by RefSeq, Jul 2008] |
| Function | Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity. [UniProt] |
| Highlight | Related products: MIF antibodies; MIF ELISA Kits; MIF Duos / Panels; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG81294 Human MIF ELISA Kit standard curve image
ARG81294 Human MIF ELISA Kit results of a typical standard run with optical density reading at 450 nm.
Specific References
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ELISA / Human
| Title | Download Link |
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| ARG81294 Human MIF ELISA Kit User's manual |
 Download
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