ARG81789
Human MICB ELISA Kit
Human MICB ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81789-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81789-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81789-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81789-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81789-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81789-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81789-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81789-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81789-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81789-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81789-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81789 Human MICB ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human MICB in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | MICB |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 31.25 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 62.5 - 4000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.2%; Inter-Assay CV: 6.7% |
| Alternate Names | MHC class I polypeptide-related sequence B; MIC-B; PERB11.2 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q29980 Human MHC class I polypeptide-related sequence B |
|---|---|
| Gene Symbol | MICB |
| Gene Full Name | MHC class I polypeptide-related sequence B |
| Background | This gene encodes a heavily glycosylated protein which is a ligand for the NKG2D type II receptor. Binding of the ligand activates the cytolytic response of natural killer (NK) cells, CD8 alphabeta T cells, and gammadelta T cells which express the receptor. This protein is stress-induced and is similar to MHC class I molecules; however, it does not associate with beta-2-microglobulin or bind peptides. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014] |
| Function | Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis. [UniProt] |
| Highlight | Related products: MICB antibodies; MICB ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Proteolytically cleaved and released from the cell surface of tumor cells. [UniProt] |
Images (1) Click the Picture to Zoom In
-
ARG81789 Human MICB ELISA Kit standard curve image
ARG81789 Human MICB ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81789 Human MICB ELISA Kit User's manual |
 Download
|
