ARG82693
Human MICA ELISA Kit
Human MICA ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82693-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82693-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82693-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82693-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82693-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82693-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82693-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82693-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82693-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82693-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82693-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82693 Human MICA ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human MICA in serum and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | MICA |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 32 pg/ml |
| Sample Type | Serum and cell culture supernatants. |
| Standard Range | 62.5 - 4000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 7.3% Inter-Assay CV: 8.1% |
| Alternate Names | MHC class I polypeptide-related sequence A; PERB11.1; MIC-A |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q29983 Human MHC class I polypeptide-related sequence A |
|---|---|
| Gene Symbol | MICA |
| Gene Full Name | MHC class I polypeptide-related sequence A |
| Background | This gene encodes the highly polymorphic major histocompatability complex class I chain-related protein A. The protein product is expressed on the cell surface, although unlike canonical class I molecules it does not seem to associate with beta-2-microglobulin. It is a ligand for the NKG2-D type II integral membrane protein receptor. The protein functions as a stress-induced antigen that is broadly recognized by intestinal epithelial gamma delta T cells. Variations in this gene have been associated with susceptibility to psoriasis 1 and psoriatic arthritis, and the shedding of MICA-related antibodies and ligands is involved in the progression from monoclonal gammopathy of undetermined significance to multiple myeloma. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2014] |
| Function | Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T-cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis. [UniProt] |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein. Cytoplasm. Note=Expressed on the cell surface in gastric epithelium, endothelial cells and fibroblasts and in the cytoplasm in keratinocytes and monocytes. Infection with human adenovirus 5 suppresses cell surface expression due to the adenoviral E3-19K protein which causes retention in the endoplasmic reticulum. [UniProt] |
| Highlight | Related products: MICA antibodies; MICA ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | N-glycosylated. Glycosylation is not essential for interaction with KLRK1/NKG2D but enhances complex formation. Proteolytically cleaved and released from the cell surface of tumor cells which impairs KLRK1/NKG2D expression and T-cell activation. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG82693 Human MICA ELISA Kit standard curve image
ARG82693 Human MICA ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82693 Human MICA ELISA Kit User manual |
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