ARG81486
Human MIA ELISA Kit
Human MIA ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81486-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81486-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81486-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81486-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81486-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81486-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81486-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81486-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81486-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81486-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81486-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81486 Human MIA ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human MIA in serum, plasma (heparin, EDTA), saliva and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | MIA |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 7.8 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA), saliva and cell culture supernatants. |
| Standard Range | 15.6 - 1000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 7.1% Inter-Assay CV: 8.0% |
| Alternate Names | CD-RAP; Melanoma inhibitory activity protein; Melanoma-derived growth regulatory protein |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q16674 Human Melanoma-derived growth regulatory protein |
|---|---|
| Gene Symbol | MIA |
| Gene Full Name | melanoma inhibitory activity |
| Function | Elicits growth inhibition on melanoma cells in vitro as well as some other neuroectodermal tumors, including gliomas. [UniProt] |
| Highlight | Related products: MIA antibodies; MIA ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | May possess two intramolecular disulfide bonds. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81486 Human MIA ELISA Kit standard curve image
ARG81486 Human MIA ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81486 Human MIA ELISA Kit User's manual |
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