ARG81521
Human MFG-E8 / Lactadherin ELISA Kit
Human MFG-E8 / Lactadherin ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81521-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81521-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81521-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81521-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81521-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81521-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81521-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81521-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81521-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81521-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81521-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81521 Human MFG-E8 / Lactadherin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human MFG-E8 / Lactadherin in serum, plasma (heparin, EDTA), saliva, urine, human milk and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | MFGE8 / Lactadherin |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 31.25 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA), saliva, urine, human milk and cell culture supernatants. |
| Standard Range | 62.5 - 4000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.5% Inter-Assay CV: 7.6% |
| Alternate Names | SPAG10; MFGM; HsT19888; HMFG; Milk fat globule-EGF factor 8; MFG-E8; BA46; hP47; EDIL1; Lactadherin; OAcGD3S; Breast epithelial antigen BA46; SED1 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | MFGE8 |
| Gene Full Name | milk fat globule-EGF factor 8 protein |
| Background | This gene encodes a preproprotein that is proteolytically processed to form multiple protein products. The major encoded protein product, lactadherin, is a membrane glycoprotein that promotes phagocytosis of apoptotic cells. This protein has also been implicated in wound healing, autoimmune disease, and cancer. Lactadherin can be further processed to form a smaller cleavage product, medin, which comprises the major protein component of aortic medial amyloid (AMA). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015] |
| Function | Plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. Promotes VEGF-dependent neovascularization (By similarity). Contributes to phagocytic removal of apoptotic cells in many tissues. Specific ligand for the alpha-v/beta-3 and alpha-v/beta-5 receptors. Also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner. Zona pellucida-binding protein which may play a role in gamete interaction. Binds specifically to rotavirus and inhibits its replication. Medin is the main constituent of aortic medial amyloid. [UniProt] |
| Highlight | Related products: MFGE8 antibodies; MFGE8 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Medin has a ragged N-terminus with minor species starting at Pro-264 and Gly-273. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81521 Human MFG-E8 / Lactadherin ELISA Kit standard curve image
ARG81521 Human MFG-E8 / Lactadherin ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81521 Human MFG-E8 / Lactadherin ELISA Kit User's manual |
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