ARG81749
Human MERTK ELISA Kit
Human MERTK ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81749-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81749-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81749-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81749-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81749-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81749-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81749-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81749-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81749-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81749-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81749-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81749 Human MERTK ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human MERTK in serum and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | MERTK |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 78 pg/ml |
| Sample Type | Serum and cell culture supernatants. |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.0%; Inter-Assay CV: 5.7% |
| Alternate Names | c-mer; Receptor tyrosine kinase MerTK; c-Eyk; Tyro12; MER; RP38; EC 2.7.10.1; Proto-oncogene c-Mer; Tyrosine-protein kinase Mer |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | MERTK |
| Gene Full Name | MER proto-oncogene, tyrosine kinase |
| Background | This gene is a member of the MER/AXL/TYRO3 receptor kinase family and encodes a transmembrane protein with two fibronectin type-III domains, two Ig-like C2-type (immunoglobulin-like) domains, and one tyrosine kinase domain. Mutations in this gene have been associated with disruption of the retinal pigment epithelium (RPE) phagocytosis pathway and onset of autosomal recessive retinitis pigmentosa (RP). [provided by RefSeq, Jul 2008] |
| Function | Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including LGALS3, TUB, TULP1 or GAS6. Regulates many physiological processes including cell survival, migration, differentiation, and phagocytosis of apoptotic cells (efferocytosis). Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with GRB2 or PLCG2 and induces phosphorylation of MAPK1, MAPK2, FAK/PTK2 or RAC1. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment. Functions in the retinal pigment epithelium (RPE) as a regulator of rod outer segments fragments phagocytosis. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3. [UniProt] |
| Highlight | Related products: MERTK antibodies; MERTK ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Autophosphorylated on Tyr-749, Tyr-753 and Tyr-754 in the activation loop allowing full activity. Autophosphorylated on Tyr-872 leading to recruitment of downstream partners of the signaling cascade such as PLCG2 (By similarity). [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81749 Human MERTK ELISA Kit standard curve image
ARG81749 Human MERTK ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81749 Human MERTK ELISA Kit User manual |
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