ARG82291
Human M-CSF ELISA Kit
Human M-CSF ELISA Kit for ELISA and Human
Overview
| Product Description | ARG82291 Human M-CSF ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human M-CSF in serum, plasma and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | Not react with Human SCF and rarely react with Mouse M-CSF. |
| Target Name | M-CSF |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 7.8 pg/ml |
| Sample Type | Serum, plasma and cell culture supernatants. |
| Standard Range | 15.6 - 1000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: < 8.5% Inter-Assay CV: < 8.5% |
| Alternate Names | Macrophage colony-stimulating factor 1; CSF-1; Lanimostim; M-CSF; MCSF |
Application Instructions
| Assay Time | ~ 3.5 hours |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P09603 Human Macrophage colony-stimulating factor 1 |
|---|---|
| Gene Symbol | CSF1 |
| Gene Full Name | colony stimulating factor 1 (macrophage) |
| Background | The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of macrophages. The active form of the protein is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. The encoded protein may be involved in development of the placenta. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2011] |
| Function | Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance. [UniProt] |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein. Processed macrophage colony-stimulating factor 1: Secreted, extracellular space. [UniProt] |
| Highlight | Related products: M-CSF antibodies; M-CSF ELISA Kits; M-CSF recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | N- and O-glycosylated. Glycosylation and proteolytic cleavage yield different soluble forms. One high molecular weight soluble form is a proteoglycan containing chondroitin sulfate. O-glycosylated with core 1 or possibly core 8 glycans. Isoform 1 is N- and O-glycosylated. Isoform 3 is only N-glycosylated. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG82291 Human M-CSF ELISA Kit standard curve image
ARG82291 Human M-CSF ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
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| ARG82291 Human M-CSF ELISA kit User's manual |
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