ARG70380
Human Lymphotoxin Alpha / TNF beta recombinant protein (Active) (His-tagged, N-ter)
Human Lymphotoxin Alpha / TNF beta recombinant protein (Active) (His-tagged, N-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged (N-ter) Active Human Lymphotoxin Alpha / TNF beta recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | Lymphotoxin alpha / TNF beta |
| Species | Human |
| A.A. Sequence | Leu1 - Leu171 |
| Expression System | E. coli |
| Protein Full Name | Lymphotoxin-alpha |
| Activity | Active |
| Activity Note | Determined by its ability to induce cytotoxicity in L929 cells in the presence of actinomycin D. The ED50 for this effect is <3 pg/mL. The specific activity of recombinant human TNF beta is > 3.3x 10^8 IU/mg. |
| Alternate Names | LTA; Lymphotoxin Alpha; TNFSF1; TNFB; LT; Tumor Necrosis Factor Ligand Superfamily Member 1; Lymphotoxin-Alpha; LT-Alpha; TNF-Beta; Lymphotoxin Alpha (TNF Superfamily, Member 1); Tumor Necrosis Factor Ligand 1E; Tumor Necrosis Factor Beta; TNF Superfamily, Member 1; TNF Superfamily Member 1; TNLG1E |
Properties
| Form | Powder |
|---|---|
| Purification Note | Endotoxin level is <0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | >98% (by SDS-PAGE) |
| Buffer | 20 mM sodium citrate and 0.2 M NaCl (pH 3.5) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not <200 μg/mL and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | LTA |
|---|---|
| Gene Full Name | Lymphotoxin Alpha |
| Background | The encoded protein, a member of the tumor necrosis factor family, is a cytokine produced by lymphocytes. The protein is highly inducible, secreted, and forms heterotrimers with lymphotoxin-beta which anchor lymphotoxin-alpha to the cell surface. This protein also mediates a large variety of inflammatory, immunostimulatory, and antiviral responses, is involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis. Genetic variations in this gene are associated with susceptibility to leprosy type 4, myocardial infarction, non-Hodgkin's lymphoma, and psoriatic arthritis. Alternatively spliced transcript variants have been observed for this gene. |
| Function | In its heterotrimeric form with LTB binds to TNFRSF3/LTBR. Lymphotoxin is produced by lymphocytes and is cytotoxic for a wide range of tumor cells in vitro and in vivo. |
| Cellular Localization | Membrane, Secreted |
| PTM | Glycoprotein |
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ARG70380 Human Lymphotoxin Alpha / TNF beta recombinant protein (Active) (His-tagged, N-ter) SDS-PAGE image
SDS-PAGE analysis of ARG70380 Human Lymphotoxin Alpha / TNF beta recombinant protein (Active) (His-tagged, N-ter)
