ARG81616
Human Lp-PLA2 ELISA Kit
Human Lp-PLA2 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81616-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81616-002 | Standard | 2 X 50 ng/vial | 4°C |
| ARG81616-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81616-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81616-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81616-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81616-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81616-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81616-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81616-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81616-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81616 Human Lp-PLA2 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Lp-PLA2 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | Lp-PLA2 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 0.39 ng/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 0.78 - 50 ng/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.0% Inter-Assay CV: 6.5% |
| Alternate Names | LDL-PLA; gVIIA-PLA2; LDL-PLA2; Group-VIIA phospholipase A2; 2-acetyl-1-alkylglycerophosphocholine esterase; EC 3.1.1.47; LDL-associated phospholipase A2; PAF 2-acylhydrolase; 2; PAF acetylhydrolase; PAFAH; Platelet-activating factor acetylhydrolase; PAFAD; 1-alkyl-2-acetylglycerophosphocholine esterase; LP-PLA2 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q13093 Human Platelet-activating factor acetylhydrolase |
|---|---|
| Gene Symbol | PLA2G7 |
| Gene Full Name | phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma) |
| Background | The protein encoded by this gene is a secreted enzyme that catalyzes the degradation of platelet-activating factor to biologically inactive products. Defects in this gene are a cause of platelet-activating factor acetylhydrolase deficiency. Two transcript variants encoding the same protein have been found for this gene.[provided by RefSeq, Dec 2009] |
| Function | Modulates the action of platelet-activating factor (PAF) by hydrolyzing the sn-2 ester bond to yield the biologically inactive lyso-PAF. Has a specificity for substrates with a short residue at the sn-2 position. It is inactive against long-chain phospholipids. [UniProt] |
| Highlight | Related products: Lp-PLA2 antibodies; Lp-PLA2 ELISA Kits; Lp-PLA2 Duos / Panels; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG81616 Human Lp-PLA2 ELISA Kit standard curve image
ARG81616 Human Lp-PLA2 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81616 Human Lp-PLA2 ELISA Kit User's manual |
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