ARG82700
Human Legumain (total) ELISA Kit
Human Legumain (total) ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82700-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82700-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82700-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82700-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82700-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82700-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82700-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82700-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82700-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82700-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82700-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82700 Human Legumain (total) ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Legumain (total) in serum, plasma (EDTA, heparin) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | Legumain (total) |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 16 pg/ml |
| Sample Type | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| Standard Range | 31.2 - 2000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.4% Inter-Assay CV: 7.1% |
| Alternate Names | PRSC1; AEP; Protease, cysteine 1; LGMN1; Asparaginyl endopeptidase; Legumain; EC 3.4.22.34 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | LGMN |
| Gene Full Name | legumain |
| Background | This gene encodes a cysteine protease that has a strict specificity for hydrolysis of asparaginyl bonds. This enzyme may be involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Enzyme activation is triggered by acidic pH and appears to be autocatalytic. Protein expression occurs after monocytes differentiate into dendritic cells. A fully mature, active enzyme is produced following lipopolysaccharide expression in mature dendritic cells. Overexpression of this gene may be associated with the majority of solid tumor types. This gene has a pseudogene on chromosome 13. Several alternatively spliced transcript variants have been described, but the biological validity of only two has been determined. These two variants encode the same isoform. [provided by RefSeq, Jul 2008] |
| Function | Has a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. Required for normal lysosomal protein degradation in renal proximal tubules. Required for normal degradation of internalized EGFR. Plays a role in the regulation of cell proliferation via its role in EGFR degradation (By similarity). May be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system. [UniProt] |
| Cellular Localization | Lysosome. [UniProt] |
| Highlight | Related products: Legumain antibodies; Legumain ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Glycosylated. Activated by autocatalytic processing at pH 4. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG82700 Human Legumain (total) ELISA Kit standard curve image
ARG82700 Human Legumain (total) ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82700 Human Legumain (total) ELISA Kit User manual |
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