ARG83321
Human LPL / Lipoprotein Lipase ELISA Kit
Human LPL / Lipoprotein Lipase ELISA Kit for ELISA and Human
Overview
| Product Description | ARG83321 Human LPL / Lipoprotein Lipase ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human LPL / Lipoprotein Lipase in Serum, Plasma and Cell culture supernatants. |
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| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | LPL / Lipoprotein Lipase |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 45 pg/ml |
| Detection Range | 78 pg/ml - 5,000 pg/ml |
| Sample Type | Serum, Plasma and Cell culture supernatants |
| Precision | Intra-Assay CV: 6.1% Inter-Assay CV: 5.7% |
| Alternate Names | EC 3.1.1.34; LPL; Lipoprotein lipase; LIPD; HDLCQ11 |
Application Instructions
| Assay Time | ~ 5 hours |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | LPL |
| Gene Full Name | lipoprotein lipase |
| Background | LPL encodes lipoprotein lipase, which is expressed in heart, muscle, and adipose tissue. LPL functions as a homodimer, and has the dual functions of triglyceride hydrolase and ligand/bridging factor for receptor-mediated lipoprotein uptake. Severe mutations that cause LPL deficiency result in type I hyperlipoproteinemia, while less extreme mutations in LPL are linked to many disorders of lipoprotein metabolism. [provided by RefSeq, Jul 2008] |
| Function | The primary function of this lipase is the hydrolysis of triglycerides of circulating chylomicrons and very low density lipoproteins (VLDL). Binding to heparin sulfate proteogylcans at the cell surface is vital to the function. The apolipoprotein, APOC2, acts as a coactivator of LPL activity in the presence of lipids on the luminal surface of vascular endothelium (By similarity). [UniProt] |
| Cellular Localization | Cell membrane; Lipid-anchor, GPI-anchor. Secreted. Note=Locates to the plasma membrane of microvilli of hepatocytes with triacyl-glycerol-rich lipoproteins (TRL). Some of the bound LPL is then internalized and located inside non-coated endocytic vesicles (By similarity). [UniProt] |
| PTM | Tyrosine nitration after lipopolysaccharide (LPS) challenge down-regulates the lipase activity. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG83321 Human LPL / Lipoprotein Lipase ELISA Kit standard curve image
ARG83321 Human LPL / Lipoprotein Lipase ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
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| ARG83321 Human LPL / Lipoprotein Lipase ELISA Kit User manual |
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