ARG81520
Human LIFR ELISA Kit
Human LIFR ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81520-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81520-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81520-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81520-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81520-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81520-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81520-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81520-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81520-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81520-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81520-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81520 Human LIFR ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human LIFR in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | LIFR |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 78 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.0% Inter-Assay CV: 6.2% |
| Alternate Names | CD118; CD antigen CD118; STWS; SJS2; LIF receptor; Leukemia inhibitory factor receptor; SWS; LIF-R |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P42702 Human Leukemia inhibitory factor receptor |
|---|---|
| Gene Symbol | LIFR |
| Gene Full Name | leukemia inhibitory factor receptor alpha |
| Background | This gene encodes a protein that belongs to the type I cytokine receptor family. This protein combines with a high-affinity converter subunit, gp130, to form a receptor complex that mediates the action of the leukemia inhibitory factor, a polyfunctional cytokine that is involved in cellular differentiation, proliferation and survival in the adult and the embryo. Mutations in this gene cause Schwartz-Jampel syndrome type 2, a disease belonging to the group of the bent-bone dysplasias. A translocation that involves the promoter of this gene, t(5;8)(p13;q12) with the pleiomorphic adenoma gene 1, is associated with salivary gland pleiomorphic adenoma, a common type of benign epithelial tumor of the salivary gland. Multiple splice variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008] |
| Function | Signal-transducing molecule. May have a common pathway with IL6ST. The soluble form inhibits the biological activity of LIF by blocking its binding to receptors on target cells. [UniProt] |
| Highlight | Related products: LIFR antibodies; LIFR ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG81520 Human LIFR ELISA Kit standard curve image
ARG81520 Human LIFR ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81520 Human LIFR ELISA Kit User's manual |
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