ARG83042
Human LIF ELISA Kit
Human LIF ELISA Kit for ELISA and Human
Overview
| Product Description | ARG83042 Human LIF ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human LIF in serum, plasma (heparin), ascites, urine and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | Not cross-reacts with: Human HGF, TGF beta1, IL6, TNF alpha. Mouse LIF, TGF beta1, IL6. Rat LIF, TGF beta1, IL6. |
| Target Name | LIF |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Protein Full Name | Leukemia inhibitory factor |
| Sensitivity | 7.8 pg/ml |
| Sample Type | Serum, plasma (heparin), ascites, urine and cell culture supernatants. |
| Standard Range | 15.6 - 1000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: less than 10% Inter-Assay CV: less than 10% |
| Alternate Names | LIF, LIF Interleukin 6 Family Cytokine, HILDA, Leukemia Inhibitory Factor, CDF, DIA, Differentiation Inhibitory Activity, Cholinergic Differentiation Factor, Differentiation Stimulating Factor, Hepatocyte Stimulating Factor III, Differentiation-Inducing Factor, Melanoma-Derived LPL Inhibitor, Human Interleukin In DA Cells, D Factor, MLPLI, LIF, Interleukin 6 Family Cytokine, Emfilermin |
Application Instructions
| Assay Time | ~ 4 hours |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | LIF |
| Gene Full Name | LIF Interleukin 6 Family Cytokine |
| Background | The protein encoded by this gene is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012] |
| Function | LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| Highlight | Related products: LIF antibodies; LIF ELISA Kits; |
| PTM | Disulfide bond, Glycoprotein. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG83042 Human LIF ELISA Kit standard curve image
ARG83042 Human LIF ELISA Kit results of standard run with optical density reading at 450 nm
| Title | Download Link |
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| ARG83042 Human LIF ELISA Kit User manual |
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