ARG81934
Human Klotho beta ELISA Kit
Human Klotho beta ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81934-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81934-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81934-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81934-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81934-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81934-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81934-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81934-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81934-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81934-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81934-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81934 Human Klotho beta ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Klotho beta in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | Klotho beta |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 78 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.9%; Inter-Assay CV: 6.7% |
| Alternate Names | BKL; Klotho beta-like protein; BetaKlotho; Beta-klotho |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | KLB |
| Gene Full Name | klotho beta |
| Function | Contributes to the transcriptional repression of cholesterol 7-alpha-hydroxylase (CYP7A1), the rate-limiting enzyme in bile acid synthesis. Probably inactive as a glycosidase. Increases the ability of FGFR1 and FGFR4 to bind FGF21 (By similarity). [UniProt] |
| Highlight | Related products: Klotho antibodies; Klotho ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG81934 Human Klotho beta ELISA Kit standard curve image
ARG81934 Human Klotho beta ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81934 Human Klotho beta ELISA Kit User manual |
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