ARG81464
Human Klk6 / neurosin ELISA Kit
Human Klk6 / neurosin ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81464-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81464-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81464-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81464-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81464-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81464-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81464-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81464-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81464-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81464-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81464-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81464 Human Klk6 / neurosin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Klk6 / neurosin in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | Klk6 / neurosin |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 39 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 78 - 5000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.1% Inter-Assay CV: 7.2% |
| Alternate Names | PRSS18; Serine protease 9; Kallikrein-6; Klk7; hK6; Neurosin; Bssp; Protease M; EC 3.4.21.-; SP59; PRSS9; Zyme; Serine protease 18 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | KLK6 |
| Gene Full Name | kallikrein-related peptidase 6 |
| Background | Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. The encoded enzyme is regulated by steroid hormones. In tissue culture, the enzyme has been found to generate amyloidogenic fragments from the amyloid precursor protein, suggesting a potential for involvement in Alzheimer's disease. Multiple alternatively spliced transcript variants that encode different isoforms have been identified for this gene. [provided by RefSeq, Jul 2008] |
| Function | Serine protease which exhibits a preference for Arg over Lys in the substrate P1 position and for Ser or Pro in the P2 position. Shows activity against amyloid precursor protein, myelin basic protein, gelatin, casein and extracellular matrix proteins such as fibronectin, laminin, vitronectin and collagen. Degrades alpha-synuclein and prevents its polymerization, indicating that it may be involved in the pathogenesis of Parkinson disease and other synucleinopathies. May be involved in regulation of axon outgrowth following spinal cord injury. Tumor cells treated with a neutralizing KLK6 antibody migrate less than control cells, suggesting a role in invasion and metastasis. [UniProt] |
| Highlight | Related products: KLK antibodies; KLK ELISA Kits; KLK Duos / Panels; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Inactivated by autolytic cleavage after Arg-80. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81464 Human Klk6 / neurosin ELISA Kit standard curve image
ARG81464 Human Klk6 / neurosin ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81464 Human Klk6 / neurosin ELISA Kit User manual |
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