ARG81487
Human Kallikrein 14 ELISA Kit
Human Kallikrein 14 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81487-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81487-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81487-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81487-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81487-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81487-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81487-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81487-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81487-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81487-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81487-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81487 Human Kallikrein 14 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Kallikrein 14 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | Kallikrein 14 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 7.8 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 15.6 - 1000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.0% Inter-Assay CV: 7.7% |
| Alternate Names | hK14; Kallikrein-like protein 6; KLK-L6; EC 3.4.21.-; Kallikrein-14 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | KLK14 |
| Gene Full Name | kallikrein-related peptidase 14 |
| Background | This gene encodes a member of the kallikrein subfamily of serine proteases that have diverse physiological functions such as regulation of blood pressure and desquamation. The altered expression of this gene is implicated in the progression of different cancers including breast and prostate tumors. The encoded protein is a precursor that is proteolytically processed to generate the functional enzyme. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015] |
| Function | Serine-type endopeptidase with a dual trypsin-like and chymotrypsin-like substrate specificity. May activate/inactivate the proteinase-activated receptors F2R, F2RL1 and F2RL3 and other kallikreins including KLK1, KLK3, KLK5 and KLK11. May function in seminal clot liquefaction through direct cleavage of the semenogelin SEMG1 and SEMG2 and activation of KLK3. May function through desmoglein DSG1 cleavage in epidermal desquamation a process by which the most superficial corneocytes are shed from the skin surface. May be involved in several aspects of tumor progression including growth, invasion and angiogenesis. [UniProt] |
| Highlight | Related products: Kallikrein antibodies; Kallikrein ELISA Kits; Kallikrein Duos / Panels; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Proteolytic cleavage of the activation peptide produces the active enzyme. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81487 Human Kallikrein 14 ELISA Kit standard curve image
ARG81487 Human Kallikrein 14 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81487 Human Kallikrein 14 ELISA Kit User's manual |
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