ARG81465
Human KLK8 ELISA Kit
Human KLK8 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81465-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81465-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81465-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81465-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81465-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81465-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81465-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81465-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81465-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81465-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81465-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81465 Human KLK8 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human KLK8 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | KLK8 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 78 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 7.0% Inter-Assay CV: 8.1% |
| Alternate Names | PRSS19; NRPN; EC 3.4.21.118; Ovasin; hK8; HNP; TADG14; Tumor-associated differentially expressed gene 14 protein; NP; Kallikrein-8; Serine protease TADG-14; Serine protease 19; Neuropsin |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | KLK8 |
| Gene Full Name | kallikrein-related peptidase 8 |
| Background | Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in tandem in a gene cluster on chromosome 19. The encoded protein may be involved in proteolytic cascade in the skin and may serve as a biomarker for ovarian cancer. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013] |
| Function | Serine protease which is capable of degrading a number of proteins such as casein, fibrinogen, kininogen, fibronectin and collagen type IV. Also cleaves L1CAM in response to increased neural activity. Induces neurite outgrowth and fasciculation of cultured hippocampal neurons. Plays a role in the formation and maturation of orphan and small synaptic boutons in the Schaffer-collateral pathway, regulates Schaffer-collateral long-term potentiation in the hippocampus and is required for memory acquisition and synaptic plasticity. Involved in skin desquamation and keratinocyte proliferation. Plays a role in the secondary phase of pathogenesis following spinal cord injury. [UniProt] |
| Highlight | Related products: KLK antibodies; KLK ELISA Kits; KLK Duos / Panels; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG81465 Human KLK8 ELISA Kit standard curve image
ARG81465 Human KLK8 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81465 Human KLK8 ELISA Kit User's manual |
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