ARG81463

Human KLK5 ELISA Kit

Human KLK5 ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG81463-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81463-002 Standard 2 X 10 ng/vial 4°C
ARG81463-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81463-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81463-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81463-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81463-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81463-008 25X Wash buffer 20 ml 4°C
ARG81463-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81463-010 STOP solution 10 ml (Ready to use) 4°C
ARG81463-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81463 Human KLK5 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human KLK5 in serum, plasma (heparin, EDTA), human milk, saliva and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name KLK5
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 39 pg/ml
Sample Type Serum, plasma (heparin, EDTA), human milk, saliva and cell culture supernatants.
Standard Range 78 - 5000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 4.8%
Inter-Assay CV: 5.4%
Alternate Names Stratum corneum tryptic enzyme; Kallikrein-like protein 2; KLKL2; KLK-L2; Kallikrein-5; EC 3.4.21.-; SCTE

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 25818 Human KLK5

Swiss-port # Q9Y337 Human Kallikrein-5

Gene Symbol KLK5
Gene Full Name kallikrein-related peptidase 5
Background Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its expression is up-regulated by estrogens and progestins. The encoded protein is secreted and may be involved in desquamation in the epidermis. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]
Function May be involved in desquamation. [UniProt]
Highlight Related products:
KLK antibodies; KLK ELISA Kits; KLK Duos / Panels;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData

Images (1) Click the Picture to Zoom In

  • ARG81463 Human KLK5 ELISA Kit standard curve image

    ARG81463 Human KLK5 ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Title Download Link
ARG81463 Human KLK5 ELISA Kit User's manual download icon Download