ARG81513
Human KLK11 ELISA Kit
Human KLK11 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81513-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81513-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81513-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81513-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81513-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81513-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81513-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81513-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81513-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81513-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81513-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81513 Human KLK11 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human KLK11 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | KLK11 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 78 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.2% Inter-Assay CV: 7.3% |
| Alternate Names | Trypsin-like protease; Kallikrein-11; hK11; PRSS20; EC 3.4.21.-; Serine protease 20; TLSP; Hippostasin |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | KLK11 |
| Gene Full Name | kallikrein-related peptidase 11 |
| Background | Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Alternate splicing of this gene results in multiple transcript variants encoding distinct isoforms which are differentially expressed.[provided by RefSeq, Nov 2009] |
| Function | Possible multifunctional protease. Efficiently cleaves 'bz-Phe-Arg-4-methylcoumaryl-7-amide', a kallikrein substrate, and weakly cleaves other substrates for kallikrein and trypsin. Cleaves synthetic peptides after arginine but not lysine residues. [UniProt] |
| Highlight | Related products: KLK antibodies; KLK ELISA Kits; KLK Duos / Panels; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | About 40% of KLK11 is inactivated by internal cleavage after Arg-188. This proteolytic inactivation may be effected by plasminogen. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81513 Human KLK11 ELISA Kit standard curve image
ARG81513 Human KLK11 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81513 Human KLK11 ELISA Kit User's manual |
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