ARG82402
Human JAM2 / JAMB / CD322 ELISA Kit
Human JAM2 / JAMB / CD322 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82402-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82402-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82402-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82402-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82402-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82402-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82402-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82402-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82402-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82402-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82402-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82402 Human JAM2 / JAMB / CD322 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human JAM2 / JAMB / CD322 in serum, plasma (EDTA, heparin) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | JAM2 / JAMB / CD322 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 10 pg/ml |
| Sample Type | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| Standard Range | 15.6 - 1000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.5% Inter-Assay CV: 6.3% |
| Alternate Names | JAM-2; Junctional adhesion molecule B; CD antigen CD322; Vascular endothelial junction-associated molecule; JAM-B; C21orf43; PRO245; JAMB; Junctional adhesion molecule 2; VEJAM; CD322; VE-JAM |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | JAM2 |
| Gene Full Name | junctional adhesion molecule 2 |
| Background | This gene belongs to the immunoglobulin superfamily, and the junctional adhesion molecule (JAM) family. The protein encoded by this gene is a type I membrane protein that is localized at the tight junctions of both epithelial and endothelial cells. It acts as an adhesive ligand for interacting with a variety of immune cell types, and may play a role in lymphocyte homing to secondary lymphoid organs. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2012] |
| Function | May play a role in the processes of lymphocyte homing to secondary lymphoid organs. [UniProt] |
| Cellular Localization | Cell junction, tight junction. Cell membrane; Single-pass type I membrane protein. Note=Localized at tight junctions of both epithelial and endothelial cells. [UniProt] |
| Highlight | Related products: JAM2 antibodies; JAM2 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG82402 Human JAM2 / JAMB / CD322 ELISA Kit standard curve image
ARG82402 Human JAM2 / JAMB / CD322 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82402 Human JAM2 / JAMB / CD322 ELISA Kit User manual |
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