ARG80109
Human IL6 (High sensitive) ELISA Kit
Human IL6 (High sensitive) ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG80109-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG80109-002 | Standard (Lyophilized) | 3 X 1 ng/vial | 4°C |
| ARG80109-003 | Standard diluent buffer | 20 ml | 4°C |
| ARG80109-004 | Antibody conjugate concentrate | 400 µl | 4°C |
| ARG80109-005 | Antibody diluent buffer | 16 ml | 4°C |
| ARG80109-006 | HRP-Streptavidin concentrate | 400 µl | 4°C (Protect from light) |
| ARG80109-007 | HRP-Streptavidin diluent buffer | 16 ml | 4°C |
| ARG80109-008 | 20X Wash buffer | 50 ml | 4°C |
| ARG80109-009 | TMB substrate | 12ml | 4°C (Protect from light) |
| ARG80109-010 | STOP solution | 12ml | 4°C |
| ARG80109-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG80109 Human IL6 (High sensitive) ELISA Kit is a high sensitive Enzyme Immunoassay kit for the quantification of Human IL6 in serum, plasma and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | No significant cross-reactivity or interference with Human CNTF, G-CSF, sgp130, IL-6 sR, OSM, IL-11, IL-12, LIF, LIF R；mouse IL-6, IL-11, IL-12；rat CNTF |
| Target Name | IL6 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm |
| Sensitivity | 0.39 pg/ml |
| Sample Type | Serum, plasma and cell culture supernatants. |
| Standard Range | 0.78 - 50 pg/ml |
| Sample Volume | 100 µl |
| Precision | CV: <9% |
| Alternate Names | B-cell stimulatory factor 2; CDF; HSF; BSF-2; Interferon beta-2; IL-6; IFNB2; CTL differentiation factor; Interleukin-6; HGF; Hybridoma growth factor; BSF2; IFN-beta-2 |
Application Instructions
| Assay Time | 4 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | IL6 |
| Gene Full Name | interleukin 6 |
| Background | This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. [provided by RefSeq, Jun 2011] |
| Function | Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T(H)17 cells. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation. [UniProt] |
| Highlight | Related products: IL6 antibodies; IL6 ELISA Kits; IL6 recombinant proteins; Related news: HMGB1 in inflammation Inflammatory Cytokines New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG80109 Human IL6 (High sensitive) ELISA Kit standard curve image
ARG80109 Human IL6 (High sensitive) ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG80109 Human IL6 (High sensitive) ELISA Kit User's manual |
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