ARG80110
Human IL6 ELISA Kit
Human IL6 ELISA Kit for ELISA and Human
Cancer kit; Immune System kit; Metabolism kit; Neuroscience kit
Publication11
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG80110-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG80110-002 | Standard (Lyophilized) | 3 X 0.2 ng/vial | 4°C |
| ARG80110-003 | Standard diluent buffer | 20 ml | 4°C |
| ARG80110-004 | Antibody conjugate concentrate | 400 µl | 4°C |
| ARG80110-005 | Antibody diluent buffer | 16 ml | 4°C |
| ARG80110-006 | HRP-Streptavidin concentrate | 400 µl | 4°C (Protect from light) |
| ARG80110-007 | HRP-Streptavidin diluent buffer | 16 ml | 4°C |
| ARG80110-008 | 20X Wash buffer | 50 ml | 4°C |
| ARG80110-009 | TMB substrate | 12ml | 4°C (Protect from light) |
| ARG80110-010 | STOP solution | 12ml | 4°C |
| ARG80110-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG80110 Human IL6 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human IL6 in serum, plasma and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | No significant cross-reactivity or interference with Human CNTF, G-CSF, sgp130, IL-6 sR, OSM, IL-11, IL-12, LIF, LIF R；mouse IL-6, IL-11, IL-12；rat CNTF |
| Target Name | IL6 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm |
| Sensitivity | 1.95 pg/ml |
| Sample Type | Serum, plasma and cell culture supernatants. |
| Standard Range | 3.125 - 200 pg/ml |
| Sample Volume | 100 µl |
| Precision | CV: less than10% |
| Alternate Names | B-cell stimulatory factor 2; CDF; HSF; BSF-2; Interferon beta-2; IL-6; IFNB2; CTL differentiation factor; Interleukin-6; HGF; Hybridoma growth factor; BSF2; IFN-beta-2 |
Application Instructions
| Assay Time | 4 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | IL6 |
| Gene Full Name | interleukin 6 |
| Background | This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. [provided by RefSeq, Jun 2011] |
| Function | Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T(H)17 cells. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation. [UniProt] |
| Highlight | Related products: IL6 antibodies; IL6 ELISA Kits; IL6 recombinant proteins; Related news: HMGB1 in inflammation Inflammatory Cytokines A new role of fat – factory of circulating miRNAs LKB1 deficiency in T cells promotes gut tumors New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Research Area | Cancer kit; Immune System kit; Metabolism kit; Neuroscience kit |
| PTM | N- and O-glycosylated. |
Images (1) Click the Picture to Zoom In
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ARG80110 Human IL6 ELISA Kit standard curve image
ARG80110 Human IL6 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
Specific References
The cytotoxicity of γδT cells in non-small cell lung cancer mediated via coordination of the BCL-2 and AKT pathways
ELISA / Human / Cell culture supernatants
IL-17C neutralization protects the kidney against acute injury and chronic injury
ELISA / Human / Cell culture supernatants
Sarcopenia is associated with mortality in non-critical elderly patients visiting the emergency department
ELISA / Human/Serum
Monoclonal enolase-1 blocking antibody ameliorates pulmonary inflammation and fibrosis
ELISA / Human / Culture supernatant
The Synergistic Inhibition of Coronavirus Replication and Induced Cytokine Production by Ciclesonide and the Tylophorine-Based Compound Dbq33b
ELISA / Human / Culture supernatant
ASC-J9 Blocks Cell Proliferation and Extracellular Matrix Production of Keloid Fibroblasts through Inhibiting STAT3 Signaling
ELISA / Human/ Cell culture supernatants
Mechanism of Huashi Xingyu Qingre recipe in treating oral lichen planus based on network pharmacology and clinical trial verification
ELISA / Human / Serum
Remdesivir and Cyclosporine Synergistically Inhibit the Human Coronaviruses OC43 and SARS-CoV-2
ELISA / Human / Cell culture supernatants
Circ_0081572 inhibits the progression of periodontitis through regulating the miR-378h/RORA axis.
ELISA / Human / Cell culture medium
Wnt5a promotes renal tubular inflammation in diabetic nephropathy by binding to CD146 through noncanonical Wnt signaling.
ELISA / Human / Cell culture supernatant
Serum and peritoneal fluid levels of interleukin-6 and interleukin-37 as biomarkers for endometriosis.
ELISA / Human
| Title | Download Link |
|---|---|
| ARG80110 Human IL6 ELISA Kit User manual |
 Download
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