ARG80176
Human IL5 ELISA Kit
Human IL5 ELISA Kit for ELISA and Human
Immune System kit
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG80176-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG80176-002 | Standard (Lyophilized) | 3 X 2 ng/vial | 4°C |
| ARG80176-003 | Standard diluent buffer | 20 ml | 4°C |
| ARG80176-004 | Antibody conjugate concentrate | 400 µl | 4°C |
| ARG80176-005 | Antibody diluent buffer | 16 ml | 4°C |
| ARG80176-006 | HRP-Streptavidin concentrate | 400 µl | 4°C (Protect from light) |
| ARG80176-007 | HRP-Streptavidin diluent buffer | 16 ml | 4°C |
| ARG80176-008 | 20X Wash buffer | 50 ml | 4°C |
| ARG80176-009 | TMB substrate | 12ml | 4°C (Protect from light) |
| ARG80176-010 | STOP solution | 12ml | 4°C |
| ARG80176-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG80176 Human IL5 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human IL5 in Serum, Plasma, Cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | No significant cross-reactivity or interference with Human GM-CS, IL-3, IL-5 R alpha, IL-5 R beta；mouse IL-5 |
| Target Name | IL5 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm |
| Sensitivity | 8 pg/ml |
| Sample Type | Serum, Plasma, Cell culture supernatants |
| Standard Range | 15.6 - 1000 pg/ml |
| Sample Volume | 100 µl |
| Precision | CV: <10% |
| Alternate Names | Eosinophil differentiation factor; EDF; IL-5; TRF; T-cell replacing factor; B-cell differentiation factor I; Interleukin-5 |
Application Instructions
| Assay Time | 4 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | IL5 |
| Gene Full Name | interleukin 5 |
| Background | Interleukin 5 (IL5) is a cytokine produced primarily by activated T lymphocytes. It exists as an antiparallel disulfide linked homodimeric glycoprotein with 115 amino-acid residues in each chain. Known also as EDF (eosinophil differentiating factor), functions predominantly as an eosinophilopoietic factor. Analysis of its crystal structure reveals a novel two-domain structure, with each domain showing significant homology to the cytokine fold in GMCSF, MCSF, IL2, IL4 and growth hormone. Human and mouse IL5 have 70% amino acid sequence homology. |
| Highlight | Related products: IL5 antibodies; IL5 ELISA Kits; IL5 recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Research Area | Immune System kit |
Images (1) Click the Picture to Zoom In
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ARG80176 Human IL5 ELISA Kit standard curve image
ARG80176 Human IL5 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG80176 Human IL5 ELISA Kit User's manual |
 Download
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