ARG83359
Human IL4 ELISA Kit
Human IL4 ELISA Kit for ELISA and Human
Cancer kit; Immune System kit
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG83359-01 | Antibody Coated Microplate | 8 X 12 strips | 4°C |
| ARG83359-02 | Standards Mixture | 3 vials | 4°C |
| ARG83359-03 | 100X Antibody Conjugate Mixture | 120 μl | ≤ -20°C |
| ARG83359-04 | 1000X HRP-Streptavidin solution | 15 μl | 4°C |
| ARG83359-05 | Standard/Sample Diluent Buffer | 20 ml | 4°C |
| ARG83359-06 | Antibody Diluent Buffer | 30 ml | 4°C |
| ARG83359-07 | 10X Wash Buffer | 50 ml | 4°C |
| ARG83359-08 | TMB substrate | 12 ml | 4°C (protect from light) |
| ARG83359-09 | STOP solution | 12 ml | 4°C |
| ARG83359-10 | Plate sealer | 4 adhesive strips | Room temperature |
Overview
| Product Description | ARG83359 Human IL4 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human IL4 in Serum, Plasma, Cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | No significant cross-reactivity or interference with IL1, IL2, IL3, IL5, IL6, IL8, IL10, IL12, IFN, TNF and sTNF-RII |
| Target Name | IL4 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm |
| Sensitivity | 3.9 pg/ml |
| Sample Type | Serum, Plasma, Cell culture supernatants |
| Standard Range | 7.8 - 500 pg/ml |
| Sample Volume | 50 µl |
| Precision | CV: <10% |
| Alternate Names | BSF-1; IL-4; B-cell stimulatory factor 1; BCGF1; Lymphocyte stimulatory factor 1; BCGF-1; Interleukin-4; Pitrakinra; Binetrakin; BSF1 |
Application Instructions
| Assay Time | 4 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | IL4 |
| Gene Full Name | interleukin 4 |
| Background | The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008] |
| Function | Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. [UniProt] |
| Highlight | Related products: IL4 antibodies; IL4 ELISA Kits; IL4 recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Research Area | Cancer kit; Immune System kit |
Images (1) Click the Picture to Zoom In
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ARG83359 Human IL4 ELISA Kit standard curve image
ARG83359 Human IL4 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG83359 Human IL4 ELISA Kit User manual |
 Download
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