ARG70082
Human IL36RN recombinant protein (Active) (His-tagged, C-ter)
Human IL36RN recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged (C-ter) Active Human IL36RN recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | IL36RN |
| Species | Human |
| A.A. Sequence | Met1 - Asp155 |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Determined by its ability to inhibit IL-36 gamma-induced IL-8 secretion in PBMC cells. The ED50 for this effect is <2 ng/mL in the presence of 500 ng/mL of recombinant human IL-36 gamma. |
| Alternate Names | IL1L1; FIL1 delta; IL-1 delta; IL-1F5; IL-1L1; Interleukin-1 receptor antagonist homolog 1; Interleukin-1-like protein 1; IL-1-related protein 3; PSORS14; IL36RA; Interleukin-1 HY1; Interleukin-1 family member 5; IL-1RP3; IL-1HY1; IL1HY1; IL1F5; IL1RP3; FIL1; IL-36Ra; FIL1D; IL-1ra homolog 1; PSORP; FIL1(DELTA); Interleukin-36 receptor antagonist protein; Interleukin-1 delta |
Properties
| Form | Powder |
|---|---|
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | >98% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | IL36RN |
|---|---|
| Gene Full Name | interleukin 36 receptor antagonist |
| Background | The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine was shown to specifically inhibit the activation of NF-kappaB induced by interleukin 1 family, member 6 (IL1F6). This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. Two alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq, Jul 2008] |
| Function | Inhibits the activity of interleukin-36 (IL36A,IL36B and IL36G) by binding to receptor IL1RL2 and preventing its association with the coreceptor IL1RAP for signaling. Part of the IL-36 signaling system that is thought to be present in epithelial barriers and to take part in local inflammatory response; similar to the IL-1 system with which it shares the coreceptor. Proposed to play a role in skin inflammation. May be involved in the innate immune response to fungal pathogens, such as Aspergillus fumigatus. May activate an anti-inflammatory signaling pathway by recruiting SIGIRR. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG70082 Human IL36RN recombinant protein (Active) (His-tagged, C-ter) SDS-PAGE image
SDS-PAGE analysis of ARG70082 Human IL36RN recombinant protein (Active) (His-tagged, C-ter).
